Local Structure in Spider Dragline Silk Investigated by Two-Dimensional Spin-Diffusion Nuclear Magnetic Resonance†
نویسندگان
چکیده
منابع مشابه
Structure of a protein superfiber: spider dragline silk.
Spider major ampullate (dragline) silk is an extracellular fibrous protein with unique characteristics of strength and elasticity. The silk fiber has been proposed to consist of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments. The repetitive sequence of a fibroin protein from major ampullate silk of the spider Nephila clavipes was determined fro...
متن کاملA two-dimensional spin-diffusion NMR study on the local structure of a water-soluble model peptide for Nephila clavipes dragline silk (MaSp1) before and after spinning
INTRODUCTION The dragline silk of the golden orb web spider Nephila clavipes has received significant attention because of its remarkable mechanical properties, which include toughness and high tensile strength.1,2 This silk contains two structural proteins designated as major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2).3,4 The dominant MaSp1 can be described as AB block...
متن کاملSecondary Structure Adopted by the Gly-Gly-X Repetitive Regions of Dragline Spider Silk
Solid-state NMR and molecular dynamics (MD) simulations are presented to help elucidate the molecular secondary structure of poly(Gly-Gly-X), which is one of the most common structural repetitive motifs found in orb-weaving dragline spider silk proteins. The combination of NMR and computational experiments provides insight into the molecular secondary structure of poly(Gly-Gly-X) segments and p...
متن کاملSupercontraction forces in spider dragline silk depend on hydration rate.
Spider dragline silk is a model biological polymer for biomimetic research due to its many desirable and unusual properties. 'Supercontraction' describes the dramatic shrinking of dragline silk fibers when wetted. In restrained silk fibers, supercontraction generates substantial stresses of 40-50 MPa above a critical humidity of approximately 70% relative humidity (RH). This stress may maintain...
متن کاملHigh-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein
Spider dragline silk is a natural fiber that has excellent tensile properties; however, it is difficult to produce artificially as a long, strong fiber. Here, the spider (Araneus ventricosus) dragline protein gene was cloned and a transgenic silkworm was generated, that expressed the fusion protein of the fibroin heavy chain and spider dragline protein in cocoon silk. The spider silk protein co...
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ژورنال
عنوان ژورنال: Macromolecules
سال: 1996
ISSN: 0024-9297,1520-5835
DOI: 10.1021/ma951098i